We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Resonance Raman spectra of the FMN of the bovine heart NADH: ubiquinone oxidoreductase, the largest membrane protein in the mitochondrial respiratory system.
- Authors
Hidehisa Sugiyama; Ryohei Nakatsubo; Satoru Yamaguchi; Takashi Ogura; Kyoko Shinzawa-Itoh; Shinya Yoshikawa
- Abstract
Abstract The resonance Raman spectra of FMN of the bovine heart NADH: ubiquinone oxidoreductase with the molecular mass of approximately one million dalton were determined by using highly improved enzyme preparation and resonance Raman apparatus. The band positions and the H2O/D2O exchange effect suggest that the N(3)−H group in the ring III of the isoalloxazine moiety is buried inside the protein to increase the vibrational coupling to the C(2)−N(3)-C(4) stretching mode and that the ring I is exposed to the aqueous phase.
- Subjects
VITAMIN B2; HEALTH of cattle; NAD(P)H dehydrogenases; RAMAN effect
- Publication
Journal of Bioenergetics & Biomembranes, 2007, Vol 39, Issue 2, p145
- ISSN
0145-479X
- Publication type
Article
- DOI
10.1007/s10863-007-9072-3