We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of continuous renal replacement therapy on renal inflammatory cytokines during extracorporeal membrane oxygenation in a porcine model.
- Authors
Hu Yimin; Yu Wenkui; Shi Jialiang; Chen Qiyi; Shen Juanhong; Lin Zhiliang; He Changsheng; Li Ning; Li Jieshou
- Abstract
Background: Extracorporeal membrane oxygenation (ECMO) has been recommended for the treatment of patients with acute, potentially reversible, life-threatening respiratory failure which unresponsive to conventional therapy. But it is unclear about how ECMO affects renal tissue. Methods: Twenty-four piglets weighing 25 to 32 kg were used in this experiment. The piglets were randomly allocated to 4 groups of 6 animals each: sham group (S group), control group (C group), VV-ECMO group (E group), VV-ECMO combined with CRRT group (EC group). The piglets were sacrificed and the kidney tissue were harvest to determine the levels of IL-1α, IL-6, TNF-α and NF-KB by using the ELISA and RT-PCR method, respectively. Results: Compared with C group and S group, E group renal tissue IL-1α, IL-6, TNF-α and NF-KB expression increased significantly, respectively (p < 0.01). Compared with E group, EC group showed renal tissue IL-1α, IL-6, TNF-α and NF-KB expression decreased significantly, respectively (p < 0.05). Conclusion: ECMO enables to inflammatory cytokines including IL-1α, IL-6, TNFα, NF-KB released significantly, renal function impaired and immune homeostasis were to imbalance; ECMO combined with CRRT treatment can alleviate levels of inflammatory cytokines, maintain immune homeostasis balance and thus ameliorate the ECMO-related acute kidney injury(AKI)
- Subjects
EXTRACORPOREAL membrane oxygenation; CYTOKINES; PIGLETS; CONTROL groups; ENZYME-linked immunosorbent assay; REVERSE transcriptase polymerase chain reaction
- Publication
Journal of Cardiothoracic Surgery, 2013, Vol 8, Issue 1, p1
- ISSN
1749-8090
- Publication type
Article
- DOI
10.1186/1749-8090-8-113