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- Title
A Role for Taok2 in Listeria monocytogenes Vacuolar Escape.
- Authors
Quereda, Juan J; Morel, Camille; Lopez-Montero, Noelia; Ziveri, Jason; Rolland, Steven; Grenier, Théodore; Aulner, Nathalie; Danckaert, Anne; Charbit, Alain; Enninga, Jost; Cossart, Pascale; Pizarro-Cerdá, Javier
- Abstract
The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
- Subjects
LISTERIA monocytogenes; SMALL interfering RNA; EPITHELIAL cells; BACTERIAL toxins; PROTEINS; LISTERIA; LISTERIOSIS; CYTOPLASM
- Publication
Journal of Infectious Diseases, 2022, Vol 225, Issue 6, p1005
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa367