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- Title
Life-span extension by dietary restriction is mediate by NLP-7 signaling and coelomocyte endocytosis in C. elegans.
- Authors
Park, Sang-Kyu; Link, Christopher D.; Johnson, Thomas E.
- Abstract
Recent studies have shown that the rate of aging can be modulated by diverse interventions. Dietary restriction is the most widely used intervention to promote longevity; however, die mechanisms underlying the effect of dietary restriction remain elusive. In a previous study, we identified two novel genes, nlp-7 and cup-4, required for normal longevity in Caenorhabditis elegans. nlp-7 is one of a set of neuropeptide-like protein genes; cup-4 encodes an ion-channel involved in endocytosis by coelomocytes. Here, we assess whether nlp-7 and cup-4 mediate longevity increases by dietary restriction. RNAi of nlp-7 or cup-4 significantly reduces die life span of the eat-2 mutant, a genetic model of dietary restriction, but has no effect on the life span of long-lived mutants resulting from reduced insulin/ IGF-1 signaling or dysfunction of the mitochondrial electron transport chain. The life-span extension observed in wild-type N2 worms by dietary restriction using bacterial dilution is prevented significantly in nlp-7 and cup-4 mutants. RNAi knockdown of genes encoding candidate receptors of NLP-7 and genes involved in endocytosis by coelomocytes also specifically shorten the life span of die eat-2 mutant. We conclude that two novel pathways, NLP-7 signaling and endocytosis by coelomocytes, are required for life extension under dietary restriction in C. elegans.
- Subjects
LONGEVITY; DIET; PROTEINS; CELLULAR signal transduction; ENDOCYTOSIS; COELOMYCETES; CAENORHABDITIS elegans; AGING prevention
- Publication
FASEB Journal, 2010, Vol 24, Issue 2, p383
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.09-142984