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- Title
Selective Loss of Calcitonin Gene--RelatedPeptide--Expressing Primary Sensory Neurons of the A-CellPhenotype in Early Experimental Diabetes.
- Authors
Yun Jiang; Nyengaard, Jens Randel; Jin Song Zhang; Jakobsen, Johannes
- Abstract
To evaluate the possible role of neuropeptide immunoreactive primary sensory neurons on the development of nociceptive dysfunction in diabetes, the absolute numbers of immunoreactive substance P and calcitonin gene-related peptide (CGRP) dorsal root ganglion (DRG) cell bodies were estimated in diabetic and non-diabetic BALB/C (p75[sup+/+]) and p75 receptor knockout (p75[sup-/-]) mice with unilateral sciatic nerve crush. The total numbers of immunoreactive substance P A-cells, substance P B-cells, CGRP A-cells, and CGRP B-cells in L5DRG were estimated using semithick consecutive sections and the optical fractionator. After 4 weeks of streptozotocin-induced diabetes, the number of immunoreactive CGRP A-cells was reduced from 692 ± 122 to 489 ± 125 (P = 0.004) in p75[sup+/+] mice on the noncrushed side. In p75[sup-/-] mice, there was no such effect of diabetes on the immunoreactive CGRP A-cell number. In p75[sup+/+] and p75[sup-/-] mice, there was no effect of diabetes on the immunoreactive CGRP B-cell number, nor was there any effect of diabetes on the immunoreactive substance P B-cell number. Sciatic nerve crush was associated with a substantial loss of L5DRG B-cells in diabetic and nondiabetic p75[sup+/+] mice and with substantial loss of immunoreactive substance P cells in diabetic p75[sup+/+] mice. In diabetic and nondiabetic p75[sup-/-] mice, there was no crush effect on neuropeptide expression. It is concluded that experimental diabetes in the mouse is associated with loss of immunoreactive CGRP primary sensory neurons of the A-cell phenotype, that this loss could play a role for the touch-evoked nociception in the model, and that the neuronal immunoreactive CGRP abnormality possibly is mediated by activation of the p75 neurotrophin receptor. Diabetes 53:2669-2675, 2004
- Subjects
NEUROPEPTIDES; SENSORY neurons; DIABETES; SUBSTANCE P; CALCITONIN gene-related peptide
- Publication
Diabetes, 2004, Vol 53, Issue 10, p2669
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/diabetes.53.10.2669