We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phytochemical screening and evaluation of cytotoxicity of stem bark extracts of Anaxagorea dolichocarpa and Duguetia chrysocarpa (Annonaceae).
- Authors
da Silva Pinheiro, Reísa; Rabelo, Suzana Vieira; de Oliveira, Ana Paula; Guimarãesxa, Amanda Leite; de Moraes-Filho, Manoel Odorico; da Costa, Marcília Pinheiro; Pessoa, Cláudia do Ó; Lúcio, Ana Sílvia Suassuna Carneiro; da Silva Almeida, Jackson Roberto Guedes
- Abstract
Purpose: To investigate the phytochemistry and cytotoxic activity of stem bark extracts from Anaxagorea dolichocarpa and Duguetia chrysocarpa - two species of the Annonaceae family. Methods: The crude ethanol bark extracts (EtOH) of the plants were obtained by maceration. The crude extracts were suspended in a mixture of methanol (MeOH) and water (H2O) (proportion 3:7 v/v) and partitioned with hexane, chloroform (CHCl3) and ethyl acetate (AcOEt) in ascending order of polarity to obtain the respective fractions. The extracts were evaluated on thin layer chromatography (TLC) plates of silica gel to highlight the main groups of secondary metabolites. Cytotoxicity was tested against human tumor cell lines - OVCAR-8 (ovarian), SF-295 (brain) and HCT-116 (colon) - using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Results: The screening results demonstrated that all the extracts were positive for the presence of flavonoids and tannins. The presence of alkaloids also was detected in some extracts. The hexane extract of A. dolichocarpa showed the strongest cytotoxicity against HCT-116 with cell growth inhibition of 89.02 %. Conclusion: The findings demonstrate for the first time the cytotoxic activity of the extracts of A. dolichocarpa and D. chrysocarpa, thus providing some evidence that plants of the Annonaceae family are a source of active secondary metabolites with cytotoxic activity.
- Subjects
BOTANICAL chemistry; CELL-mediated cytotoxicity; ANNONACEAE; THIN layer chromatography; CHLOROFORM; METABOLITES
- Publication
Tropical Journal of Pharmaceutical Research, 2016, Vol 15, Issue 4, p793
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v15i4.18