We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential effects of azathioprine on T cells regulating murine B-cell function.
- Authors
Duclos, Hélène; Maillot, Marie-Christine; Galanaud, P.
- Abstract
We describe selective effects of azathioprine (Az) on T-cell subpopulations regulating the primary in vitro antibody response of mouse spleen cells to the T-independent antigen TNP-polyacrylamide. This response is susceptible to the effect of two kinds of non-specific suppressor cells: (i) spontaneously- induced suppressors, generated after 4–5 days culture in the absence of antigen; (ii) mitogen-induced sup- pressors, produced after 2 days in culture in the presence of 2 μg/ml of concanavalin A (Con A). Indeed, both these precultured cells lead to a cell dose-dependent suppression of the anti-TNP response when transferred at the initiation of antigen-stimulated fresh cell cultures. T cells are the effectors of both these suppressions and seem to directly suppress the B-cell response. We tested the in vitro effect of Az (10-1 μg/ml) on the generation of these two sets of suppressors. Whereas that of Con-A-induced suppressors proves to be resistant, that of spontaneously-induced T suppressors is totally prevented by the addition of Az in the preculture medium. Instead, Az treatment allows the manifestation of a spontaneously-induced helper T cell, simultaneously generated, which is able to increase a T-independent antibody response and quite resistant to the in vitro effect of Az. Thus, this study demonstrates that different subpopulations of T lymphocytes regulating the B-cell antibody response exhibit a selective sensitivity to Az, implying different cell proliferation requirements and/or different cellular origin.
- Subjects
IMMUNOLOGY; B cell differentiation -- Molecular aspects; T cells; ANTIGEN-antibody reactions; CELL proliferation; IMMUNE response
- Publication
Immunology, 1982, Vol 46, Issue 3, p595
- ISSN
0019-2805
- Publication type
Article