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- Title
Long-lasting Effect Against White Spot Syndrome Virus in Shrimp Broodstock, Litopenaeus vannamei, by LvRab7 Silencing.
- Authors
Alvarez‐Ruiz, Píndaro; Luna‐González, Antonio; Escamilla‐Montes, Ruth; Mejía‐Ruiz, Claudio H.; Magallón‐Barajas, Francisco J.; Llera‐Herrera, Raúl; Galván‐Alvarez, Diego A.
- Abstract
The white spot syndrome virus ( WSSV) remains the most devastating viral pathogen of shrimp culture worldwide. Gene silencing by RNA interference ( RNAi) using double stranded RNA ( dsRNA) has been considered a powerful tool for conferring protection against WSSV when viral genes are silenced, as documented in several shrimp species. However, this effect is not long lasting. Our results provide the first evidence that long-term silencing of the LvRab7 endogen produced antiviral effect against WSSV, which endured at least 21 d after dsRNA treatment (dat). Until now, the most efficient way to implement RNAi with dsRNA into the shrimp is by injection. Consequently, its application to broodstock in hatcheries is possible, minimizing the risk of vertical transmission of the virus. We show that the expression of Rab7 in hemocytes is lowest at 2 dat and finally recovers to basal status. In contrast, in gills and pleopods, gene expression silencing continued for at least 21 d. We challenged Litopenaeus vannamei broodstock with WSSV at 7, 14, or 21 dat reaching mortality rates of 0, 40, and 27%, respectively. In conclusion, the LvRab7 gene silencing is progressive and effective against WSSV. However, further studies are necessary to elucidate the functions of Rab7 in shrimp cells before applying this methodology at a commercial level.
- Subjects
LITOPENAEUS; BROOD stock assessment; SHRIMPS; WHITE spot syndrome virus; PATHOGENIC microorganisms; PHYSIOLOGY
- Publication
Journal of the World Aquaculture Society, 2015, Vol 46, Issue 6, p571
- ISSN
0893-8849
- Publication type
Article
- DOI
10.1111/jwas.12236