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- Title
Chemical composition, antinociceptive, antiinflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. (Cyperaceae).
- Authors
Rabelo, Alessandra Silva; Serafini, Mairim Russo; Rabelo, Thallita Kelly; de Melo, Marcelia Garcez Dória; da Silva Prado, Douglas; Gelain, Daniel Pens; Fonseca Moreira, José Claudio; Santos Bezerra, Marília dos; da Silva, Thanany Brasil; Costa, Emmanoel Vilaça; de Lima Nogueira, Paulo Cesar; de Souza Moraes, Valéria Regina; do Nascimento Prata, Ana Paula; Quintans, Jr., Lucindo José; Souza Araújo, Adriano Antunes
- Abstract
Background The present study was carried out to evaluate antioxidant, antinociceptive and antiinflammatory activities of essential oil from R. maritima (RMO) in experimental protocols. Methods The essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography/mass spectrometry (GC/MS). Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS). NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test. Results The majors compounds identified was remirol (43.2%), cyperene (13.8%), iso-evodionol (5.8%), cyperotundone (5.7%), caryophyllene oxide (4.9%), and rotundene (4.6%). At the TRAP assay, RMO concentration of 1 mg.mL-1 showed anti-oxidant effects and at concentration of 1 and 10 ng.mL-1 RMO showed pro-oxidant effect. RMO at 1 mg.mL-1 also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL-1 to 100 μg.mL-1 enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL-1 to 100 μg.mL-1. We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally (p.o.) administered significantly inhibited (p < 0.05, p < 0.01 and p < 0.001) the acetic acid-induced writhings and two phases of formalin-induced nociception in mice. Conclusion The RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.
- Subjects
ANALGESICS; ANALYSIS of variance; ANIMAL experimentation; ANTI-inflammatory agents; ANTIOXIDANTS; BIOLOGICAL assay; CHEMICAL reagents; CHEMILUMINESCENCE assay; ESSENTIAL oils; GAS chromatography; MASS spectrometry; LIPID peroxidation (Biology); MICE; MOLECULAR structure; OXIDATION-reduction reaction; PAIN; RESEARCH funding; STATISTICS; PLANT extracts; DATA analysis; PAIN measurement; DATA analysis software; FREE radical scavengers; DESCRIPTIVE statistics; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2014, Vol 14, Issue 1, p1031
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-14-514