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- Title
Prevention Effect in Selenite-Induced Cataract in Vivo and Antioxidative Effects in Vitro of Crataegus pinnatifida Leaves.
- Authors
Tao Wang; Peng Zhang; Chunfeng Zhao; Yi Zhang; Hong Liu; Limin Hu; Xiumei Gao; Deqin Zhang
- Abstract
Cataract is the leading cause of blindness worldwide. It is a multifactorial disease primarily associated with oxidative stress produced by free radicals. The present study was undertaken to evaluate the anticataract potential of Crataegus pinnatifida (hawthorn tree) leaves extract in selenite-induced cataract in vivo and antioxidant effects in vitro. In vitro antioxidant assay of C. pinnatifida leaves extract on NO production inhibition, aldose reductase inhibition, and O radical scavenging activities gave the IC of 98.3, 89.7, and 5.98 μg/mL, respectively. To characterize some major compounds in C. pinnatifida leaves extract, nine flavonoids were identified via LC-MS/MS qualitative analysis. Based on in vitro screening results, C. pinnatifida leaves extract eye drops in 0.1% hydroxypropyl methyl cellulose solution were prepared to evaluate the anticataract potential in vivo. Administration of C. pinnatifida leaves extract eye drops alternately three times a day in rat pups with selenite-induced oxidative stress significantly increased serum SOD and CAT activities, and tended to reduce MDA level compared with control group. The antioxidant enzyme SOD, CAT, and GSH activities in lens showed a significant increase. These results may be applied in the future for the prevention and treatment of cataracts.
- Subjects
CATARACT; SELENITES; CRATAEGUS pinnatifida; FLAVONOIDS; OXIDATIVE stress; ALDOSE reductase; FREE radicals
- Publication
Biological Trace Element Research, 2011, Vol 142, Issue 1, p106
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-010-8752-8