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- Title
Black Sorghum Phenolic Extract Regulates Expression of Genes Associated with Oxidative Stress and Inflammation in Human Endothelial Cells.
- Authors
Francis, Nidhish; Rao, Shiwangini; Blanchard, Christopher; Santhakumar, Abishek; Castell Escuer, Margarida; Camps-Bossacoma, Mariona
- Abstract
Oxidative stress is one of the primary factors leading to endothelial dysfunction, a major underlying cause of vascular disorders. This study aims to understand the key signalling pathways regulated by sorghum (Shawaya short black 1 variety; characterised to be very high in its antioxidant activity) under oxidative stress in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were pre-treated with non-cytotoxic concentrations of phenolic-rich black sorghum extract (BSE) prior to induction of oxidative stress using hydrogen peroxide (H2O2). Treatment with BSE upregulated the expression of heme oxygenase 1 (HO1) and endothelial nitric oxide synthase (eNOS) and downregulated the levels of NADPH oxidase 4 (NOX4). BSE treatment significantly reduced the expression of pro-inflammatory mediators such as monocyte chemoattractant protein 1 (MCP1) and intracellular adhesion molecule 1 (ICAM1). Results from this study suggest that phenolic-rich BSE may reduce oxidative stress by regulating pro- and antioxidant signalling pathways and the expression of inflammatory mediators linked to endothelial dysfunction under oxidative stress.
- Subjects
OXIDATIVE stress; ENDOTHELIAL cells; MONOCYTE chemotactic factor; SORGHUM; NITRIC-oxide synthases; BOVINE spongiform encephalopathy
- Publication
Molecules, 2019, Vol 24, Issue 18, p3321
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules24183321