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- Title
Morusin Functions as a Lipogenesis Inhibitor as Well as a Lipolysis Stimulator in Differentiated 3T3-L1 and Primary Adipocytes.
- Authors
Lee, Mi Rim; Kim, Ji Eun; Choi, Jun Young; Park, Jin Ju; Kim, Hye Ryeong; Song, Bo Ram; Park, Ji Won; Kang, Mi Ju; Hwang, Dae Youn; Choi, Young Whan; Kim, Kyung Mi
- Abstract
Conflicting results for morusin activity during adipogenic differentiation are reported in 3T3-L1 adipocytes and cancer cells. To elucidate the influence of morusin on fat metabolism, their anti-obesity effects and molecular mechanism were investigated in 3T3-L1 cells and primary adipocytes. Morusin at a dose of less than 20 µM does not induce any significant change in the viability of 3T3-L1 adipocytes. The accumulation of intracellular lipid droplets in 3T3-L1 adipocytes stimulated with 0.5 mM 3-isobutyl-1-methylxanthine, 1 µM dexamethasone, 10 µg/mL insulin in DMEM containing 10% FBS (MDI)-significantly reduces in a dose-dependent manner after morusin treatment. The phosphorylation level of members in the MAP kinase signaling pathway under the insulin receptor downstream also decrease significantly in the MDI + morusin-treated group compared to MDI + vehicle-treated group. Also, the expression of adipogenic transcription factors (<italic>PPARγ</italic> and <italic>C/EBPα</italic>) and lipogenic proteins (<italic>aP2</italic> and <italic>FAS</italic>) are significantly attenuated by exposure to the compound in MDI-stimulated 3T3-L1 adipocytes. Furthermore, the decrease in the G0/G1 arrest of cell cycle after culturing in MDI medium was dramatically recovered after co-culturing in MDI + 20 µM morusin. Moreover, morusin treatment induces glycerol release in the primary adipocytes of SD rats and enhances lipolytic protein expression (HSL, ATGL, and perilipin) in differentiated 3T3-L1 adipocytes. Overall, the results of the present study provide strong evidence that morusin inhibits adipogenesis by regulating the insulin receptor signaling, cell cycle and adipogenic protein expression as well as stimulating lipolysis by enhancing glycerol release and lipolytic proteins expression.
- Subjects
ANTINEOPLASTIC agents; LIPID synthesis; LIPOLYSIS; FAT cells; CANCER cells; CELL differentiation
- Publication
Molecules, 2018, Vol 23, Issue 8, p2004
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23082004