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- Title
Optimization of Ultrasound-Assisted Extraction, HPLC and UHPLC-ESI-Q-TOF-MS/MS Analysis of Main Macamides and Macaenes from Maca (Cultivars of Lepidium meyenii Walp).
- Authors
Shu-Xiao Chen; Ke-Ke Li; Duoji Pubu; Si-Ping Jiang; Bin Chen; Li-Rong Chen; Zhen Yang; Chao Ma; Xiao-Jie Gong
- Abstract
Ultrasound-assisted extraction (UAE), using petroleum ether as the solvent, was systematically applied to extract main macamides and macaenes from Maca hypocotyls. Extraction yield was related with four variables, including ratio of solution to solid, extraction temperature, extraction time, and extraction power. On the basis of response surface methodology (RSM), the optimal conditions were determined to be the ratio of solution to solid as 10:1 (mL/g), the extraction temperature of 40 °C, the extraction time of 30 min, and the extraction power of 200W. Based on the optimal extraction method of UAE, the total contents of ten main macamides and two main macaenes of Maca cultivated in twenty different areas of Tibet were analyzed by HPLC and UHPLC-ESI-Q-TOF-MS/MS. This study indicated that UAE was able to effectively extract macamides alkaloids from Maca hypocotyls. Quantitative analysis showed that geographical origins, not ecotypes, played a more important role on the accumulation of active macamides in Maca.
- Subjects
MACA (Plant); PLANT extracts; EXTRACTION (Chemistry); HIGH performance liquid chromatography; HYPOCOTYLS; ALKALOIDS
- Publication
Molecules, 2017, Vol 22, Issue 12, p2196
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22122196