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- Title
A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells.
- Authors
Chao Huang; Zhongkai Zhu; Xiyue Cao; Xingfu Chen; Yuping Fu; Zhengli Chen; Lixia Li; Xu Song; Renyong Jia; Zhongqiong Yin; Gang Ye; Bin Feng; Yuanfeng Zou
- Abstract
Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications.
- Subjects
POLYSACCHARIDES; PECTIC enzymes; ANTIOXIDANTS; SPLEEN diseases; GASTROINTESTINAL diseases; OXIDATIVE stress
- Publication
Molecules, 2017, Vol 22, Issue 8, p1341
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22081341