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- Title
An Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC.
- Authors
Lei Wen; Yunliang Lin; Ruimin Lv; Huijiao Yan; Jinqian Yu; Hengqiang Zhao; Xiao Wang; Daijie Wang
- Abstract
In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (-)-epicatechin (1), quercetin-3-O-(2,6-di-α-L-rhamnopyranosyl)-β-D-galactopyranoside (2), 4''-O-glucosylvitexin (3) and 2''-O-rhamnosylvitexin (4) as well as a mixture of three further flavonoids. An extrusion mode was used to rapidly separate quercetin-3-O-(2,6-di-α-L-rhamnopyranosyl)-β-D-galactopyranoside with a big KD-value. In the second step, the mixture that resulted from HSCCC was separated by pre-HPLC, resulting in three pure compounds including: vitexin (5), hyperoside (6) and isoquercitrin (7). The purities of the isolated compounds were established to be over 98%, as determined by HPLC. The structures of these seven flavonoids were elucidated by ESI-MS and NMR spectroscopic analyses.
- Subjects
FLAVONOIDS; CRATAEGUS pinnatifida; NUCLEAR magnetic resonance; CHLOROFORM; ISOQUERCITRIN
- Publication
Molecules, 2017, Vol 22, Issue 5, p767
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22050767