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- Title
Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment.
- Authors
García, Jorge López; Asadinezhad, Ahmad; Pacherník, Jirí; Lehocký, Marián; Junkar, Ita; Humpolícek, Petr; Sáha, Petr; Valášek, Pavel
- Abstract
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
- Subjects
PLASMA chemistry; CELL proliferation; CELL growth; ARGON spectra; ARGON plasmas; CHEMICAL reactions; INFRARED spectroscopy; SPECTRUM analysis; BIOCHEMISTRY; CHEMICAL biology
- Publication
Molecules, 2010, Vol 15, Issue 4, p2845
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules15042845