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- Title
Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.
- Authors
Ujihara, Takako; Uchiyama, Jumpei; Nasukawa, Tadahiro; Ando, Hiroki; Murakami, Hironobu; Ohara, Naoya; Ogawa, Midori; Yamazaki, Toshio; Daibata, Masanori; Sakaguchi, Masahiro; Matsuzaki, Shigenobu
- Abstract
Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
- Subjects
BACTERIOPHAGES; MYCOBACTERIA; BACTERIAL genes; POLYMERASE chain reaction; SIPHOVIRIDAE; FREEZE-drying; BACTERIAL genetics
- Publication
Archives of Virology, 2018, Vol 163, Issue 7, p1915
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-018-3788-8