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- Title
The construction and application of a cell line resistant to novel subgroup avian leukosis virus (ALV-K) infection.
- Authors
Mingzhang, Rao; Zijun, Zhao; Lixia, Yuan; Jian, Chen; Min, Feng; Jie, Zhang; Ming, Liao; Weisheng, Cao
- Abstract
A novel avian leukosis viruses (ALV) subgroup named ALV-K was recently isolated from Chinese indigenous chickens which is different from the subgroups (A to E and J) that have previously been reported to infect chickens. More and more ALV-K strains have recently been isolated from local breeds of Chinese chickens. However, there are no more effective diagnostic methods for ALV-K other than virus isolation followed by envelope gene sequencing and comparison. Viral infection can be blocked through expression of the viral receptor-binding protein. In this study, we have engineered a cell line, DF-1/K, that expresses ALV-K env protein and thereby confers resistance to ALV-K infection. DF-1/K can be used in combination with the ALV-K susceptible cell line DF-1 as a specific diagnostic tool for ALV-K and provides a good tool for further research into the molecular mechanisms of interaction between ALV-K env protein and the host cell receptor.
- Subjects
AVIAN leukosis; VIRAL receptors; GENE expression; CELL lines; CHICKEN diseases; DIAGNOSIS
- Publication
Archives of Virology, 2018, Vol 163, Issue 1, p89
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-017-3563-2