We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antioxidative enzymes, calcium, and ABA signaling pathway are required for the stress tolerance of transgenic wheat plant by the ectopic expression of harpin protein fragment Hpa1 under heat stress.
- Authors
Wang, D.; Pang, X.; Yang, F.; Kou, L.; Zhang, X.; Yu, P.; Niu, Y.
- Abstract
Genetic engineering for heat stress tolerance can promote crop growth and improve yield. One wheat (Triticum aestivum L.) line Y16 (wild type) and two transgenic plants (Y16-3 and Y16-46) that express Hpa1, a functional fragment of harpin protein, were used in this study to investigate their possible abiotic stress tolerance under heat stress. Results showed that enhanced thermotolerance was observed in the Y16-3 and Y16-46 lines over the control wheat under stress conditions. However, this increased stress tolerance was significantly abolished by specific inhibitors such as fluridone or sodium tungstate (i.e., arrests abscisic acid (ABA) biosynthesis) and EGTA or La (i.e., arrests Ca signaling pathway) under heat exposure. By contrast, high activities of antioxidant enzymes such as superoxide dismutase, catalase, and ascorbate peroxidase (but not peroxidase) and low levels of oxidative damage (thiobarbituric acid reactive substance (TBARS) and chlorophyll) were detected in transgenic wheat lines compared with the control plant under stress exposure. However, this significant difference diminished after the addition of these specific inhibitors. Furthermore, a slight increase of HO was observed in the transgenic plant, instead of the control, without the addition of chemicals under heat stress. These results suggested that antioxidant enzymes, calcium, and ABA signaling pathways were involved in this Hpa1-mediated thermotolerance of transgenic wheat plants under stress exposure. Finally, a hypothetical model based on HO signaling was proposed to illustrate the possible mechanism of this enhanced heat stress tolerance.
- Subjects
GENETIC engineering of crops; CROP improvement; ABIOTIC stress; HARPINS; FLURIDONE; ANTIOXIDANTS; TRANSGENIC plants
- Publication
Russian Journal of Plant Physiology, 2017, Vol 64, Issue 6, p899
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443717060140