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- Title
Production of d-arabitol from raw glycerol by Candida quercitrusa.
- Authors
Yoshikawa, Jun; Habe, Hiroshi; Morita, Tomotake; Fukuoka, Tokuma; Imura, Tomohiro; Iwabuchi, Hiroyuki; Uemura, Shingo; Tamura, Takamitsu; Kitamoto, Dai
- Abstract
To promote the effective use of raw glycerol (a by-product of biodiesel production), 110 yeast strains that produce d-arabitol from glycerol were isolated from environmental samples. Among them, strain 17-2A was an effective d-arabitol producer in the presence of 250 g/l glycerol and was identified as Candida quercitrusa based on morphological, physicochemical, and phylogenetic analyses. C. quercitrusa type strain NBRC1022 produced the greatest quantity of d-arabitol (41.7 g/l) when the ability to produce d-arabitol from raw glycerol was compared among C. quercitrusa 17-2A and its phylogenetically related strains in flask culture. Under optimized culture conditions, strain NBRC1022 produced d-arabitol at a concentration of 58.2 g/l after a 7-day cultivation in 250 g/l glycerol, 6 g/l yeast extract, and 2 g/l CaCl. The culture conditions were further investigated with raw glycerol using a jar fermenter; the concentration of d-arabitol reached 67.1 g/l after 7 days and 85.1 g/l after 10 days, respectively, which corresponded to 0.40 g/g of glycerol. To our knowledge, the present d-arabitol yield from glycerol is higher than reported previously using microbial production.
- Subjects
ARABITOL; GLYCERIN; CANDIDA; BIODIESEL fuels; YEAST extract; FUNGAL cultures
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 7, p2947
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-5449-x