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- Title
Growth inhibition of biomass adapted to the degradation of toluene and xylenes in mixture in a batch reactor with substrates supplied by pulses
- Authors
Seignez, Chantal; Peringer, Paul; Leon, Elizabeth; Adler, Nevenka
- Abstract
A biomass adapted to degrade toluene and xylenes in mixture was grown in a batch reactor with substrates supplied by pulses. The inhibition of biomass growth in the course of substrate degradation was investigated. The maximal biomass concentration of 7 g l-1 was obtained using 150 microl of toluene and 15 microl of a mixture of xylenes in one litre of liquid medium, and the maximal biomass productivity and yield were 53 mg l-1 h-1 and 0.32 gDW gs-1, respectively. Higher quantities of substrate added by pulses, that is 200 microl of toluene with 20microl of xylenes and 300 microl of toluene with 30 microl of xylenes, caused an accumulation of metabolites. These higher quantities of substrates caused inhibition of microbial growth. Among the metabolites produced, 4-methyl catechol was found in large quantities in the culture medium and in the cells.
- Subjects
BIODEGRADATION; WASTE treatment
- Publication
Biodegradation, 1999, Vol 10, Issue 4, p245
- ISSN
0923-9820
- Publication type
Article
- DOI
10.1023/a:1008363422254