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- Title
Potential antimicrobial and antiadhesive activity of crude biosurfactants produced by Leuconostoc sp strains coated on rubbersilicone tubes.
- Authors
Bellil, Yahia; Bellil, Wassila Chahrour; Benmechernene, Zineb; Kihal, Mebrouk
- Abstract
The present study focused on screening of biosurfactants produced by Leuconostoc strains isolated from dromedary milk with antimicrobial and antiadhesive properties against various pathogenic microorganisms and its potential application in medical fields as an antibiofilm agent. After identification of the selected strains by mass spectrometry, screening methods for biosurfactants production were used, including drop collapse, oil displacement, surface tension and emulsification index. Antiadhesive assays were carried out against six pathogenic microorganisms in polystyrene surface, and applied on rubber silicone tubes. Four lactic acid bacteria were isolated and identified as Leuconostoc sp according to MALDITOF MS spectrometry. Biosurfactants isolated from Leuconostoc strains reduced tension surface from 72.0 to 45.7 by strain BY14, with an emulsification efficiency (E24) after 24h of 63%. The crude biosurfactants were effective against most pathogenic microorganisms with inhibition percentage from 92.3% to 44.3% after adhesion to polystyrene. Otherwise, the antiadhesive activity displayed values from 75.67% to 13.2%. The result of application test demonstrated that the selected crude biosurfactants reduced significantly all the deposited microbial biofilm from rubber silicone tubes. This study suggest that biosurfactants could have a promising role as an effective antibiofilm to prevent microbial colonization on medical devices.
- Subjects
LEUCONOSTOC; SILICONE rubber; LACTIC acid bacteria; BIOSURFACTANTS; COLONIZATION (Ecology); SURFACE tension; GLYCOLIPIDS
- Publication
South Asian Journal of Experimental Biology, 2021, Vol 11, Issue 2, p106
- ISSN
2230-9799
- Publication type
Article
- DOI
10.38150/sajeb.11(2).p106-114