We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Local Enrichment with Convergence of Enriched T-Cell Clones Are Hallmarks of Effective Peptide Vaccination against B16 Melanoma.
- Authors
Izosimova, Anna Vyacheslavovna; Shabalkina, Alexandra Valerievna; Myshkin, Mikhail Yurevich; Shurganova, Elizaveta Viktorovna; Myalik, Daria Sergeevna; Ryzhichenko, Ekaterina Olegovna; Samitova, Alina Faritovna; Barsova, Ekaterina Vladimirovna; Shagina, Irina Aleksandrovna; Britanova, Olga Vladimirovna; Yuzhakova, Diana Vladimirovna; Sharonov, George Vladimirovich
- Abstract
Background: Some peptide anticancer vaccines elicit a strong T-cell memory response but fail to suppress tumor growth. To gain insight into tumor resistance, we compared two peptide vaccines, p20 and p30, against B16 melanoma, with both exhibiting good in vitro T-cell responses but different tumor suppression abilities. Methods: We compared activation markers and repertoires of T-lymphocytes from tumor-draining (dLN) and non-draining (ndLN) lymph nodes for the two peptide vaccines. Results: We showed that the p30 vaccine had better tumor control as opposed to p20. p20 vaccine induced better in vitro T-cell responsiveness but failed to suppress tumor growth. Efficient antitumor vaccination is associated with a higher clonality of cytotoxic T-cells (CTLs) in dLNs compared with ndLNs and the convergence of most of the enriched clones. With the inefficient p20 vaccine, the most expanded and converged were clones of the bystander T-cells without an LN preference. Conclusions: Here, we show that the clonality and convergence of the T-cell response are the hallmarks of efficient antitumor vaccination. The high individual and methodological dependencies of these parameters can be avoided by comparing dLNs and ndLNs.
- Subjects
PEPTIDE vaccines; T cells; PEPTIDES; MELANOMA; VACCINATION; T cell receptors; BCG vaccines
- Publication
Vaccines, 2024, Vol 12, Issue 4, p345
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines12040345