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- Title
[ NSI] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes.
- Authors
Nizhnikov, Anton; Magomedova, Zalina; Rubel, Alexandr; Kondrashkina, Alexandra; Inge-Vechtomov, Sergey; Galkin, Alexey
- Abstract
We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [ NSI] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [ NSI] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [ NSI] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [ NSI], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [ NSI] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [ NSI] and [ nsi] strains. We demonstrate that VTS1 overexpression, like [ NSI] induction, causes translational read-through and growth defects in S. cerevisiae.
- Subjects
RNA; GENE expression; GENETIC code; GENETIC translation; CARRIER proteins; NONSENSE suppression (Genetics); YEAST
- Publication
Current Genetics, 2012, Vol 58, Issue 1, p35
- ISSN
0172-8083
- Publication type
Article
- DOI
10.1007/s00294-011-0363-1