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- Title
人参皂苷 Rh2 对大鼠 C6 胶质瘤细胞 Siah-1、Synaptophysin、MMP9 及 VEGF 表达的影响.
- Authors
吴国玉; 杨 俊; 熊绍权; 祝 捷; 李建梅
- Abstract
Objective: To investigate the impacts of ginsenoside Rh2 on the Siah-1, synaptophysin, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) expression in rat C6 glioma cells. Methods: Rat C6 glioma cells were grouped into control group, low-dose ginsenoside Rh2 group (16 μg/mL), medium dose ginsenoside Rh2 group (32 μg/mL), and high-dose ginsenoside Rh2 group (48 μg/mL), cell proliferation was detected by CCK-8 method and plate cloning test. Cell apoptosis was detected by flow cytometry. Cell invasion was detected by Transwell. The VEGF, Siah-1, Synaptophysin and MMP-9 mRNA expression in C6 glioma cells was detected by real-time fluorescence quantitation-polymerase chain reaction (qRT-PCR); and Western blot was used to detect the protein VEGF, Siah-1, Synaptophysin and MMP-9 expression in C6 glioma cells. Results: Compared with the control group, the OD450 value (24 h, 48 h), clone formation rate, cell invasion number, VEGF, Synaptophysin, MMP-9 mRNA and protein expression in C6 glioma cells in the low-dose ginsenoside Rh2 group, the medium dose ginsenoside Rh2 group, and the high-dose ginsenoside Rh2 group were decreased, the cell apoptosis rate and the Siah-1 mRNA and protein expression were increased, which were dose dependent (P<0.05). Conclusions: Ginsenoside Rh2 may inhibit the proliferation and invasion of rat C6 glioma cells and promote cell apoptosis by up-regulating Siah-1 and down-regulating the VEGF, Synaptophysin and MMP-9 expression.
- Subjects
VASCULAR endothelial growth factors; GINSENOSIDES; GENE expression; MOLECULAR cloning; SYNAPTOPHYSIN
- Publication
Progress in Modern Biomedicine, 2023, Vol 23, Issue 13, p2419
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2023.13.004