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- Title
Synthesis of Glutathione (GSH)‐Responsive Amphiphilic Duplexes and their Application in Gene Delivery.
- Authors
Li, Yong‐Qiang; Sun, Wan; Liu, Xu‐Ying; Chen, Li‐Qing; Huang, Wei; Lu, Zhong‐Lin; He, Lan
- Abstract
Oligoamide molecular strands with hydrogen‐bonding sequences DADDAD and guanidine (O‐1) or 1,5,9‐triazacyclododecane ([12]aneN3; O‐2) side chains and oligoamides with hydrogen‐bonding sequences ADAADA and octyl moieties (O‐3), were synthesized. Two duplexes (D‐1 and D‐2) were prepared by conjugating the hydrophilic O‐1 or O‐2 and hydrophobic O‐3 through sequence‐specific hydrogen‐bond association and cross‐linked disulfide bonds. Electrophoresis measurements indicated that O‐1, O‐2, D‐1, and D‐2 were able to completely retard the DNA mobiliy at concentrations of 30, 30, 10, and 20 μM, respectively. Reversible DNA release in O‐1 and O‐2 complexes can be achieved in the presence of heparin sodium, whereas the presence of GSH greatly improved DNA release in D‐1 and D‐2 complexes. The particles formed were in a size range of 50–170 nm with positively charged surfaces. D‐1 and D‐2 transfected pEGFP−N1 into HeLa cells successfully.
- Subjects
GLUTATHIONE; HELA cells; GENES; DNA; GUANIDINES; HEPARIN; GLUTATHIONE peroxidase
- Publication
ChemPlusChem, 2019, Vol 84, Issue 8, p1060
- ISSN
2192-6506
- Publication type
Article
- DOI
10.1002/cplu.201900295