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- Title
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories.
- Authors
Kesterson, Alexandria E.; Craig, John E.; Chuvala, Lara J.; Heine, Henry S.
- Abstract
The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.
- Subjects
HAZARDOUS substance safety measures; BURKHOLDERIA; GRAM-negative bacteria; LABORATORIES; BACILLUS (Bacteria); STERILIZATION (Disinfection)
- Publication
Emerging Infectious Diseases, 2020, Vol 26, Issue 11, p2586
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2611.191630