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- Title
Proteomic Analysis of Effects on Natural Herb Additive Containing Immunoglobulin Yolksac (IgY) in Pigs.
- Authors
Park, Hyeon-Soo; Park, Kwang-Il; Nagappan, Arulkumar; Lee, Do-Hoon; Kang, Sang-Rim; Kim, Jin-A; Kim, Eun Hee; Han, Dae-Yong; Kim, Gon-Sup
- Abstract
Thirty male pigs were infected orally with E. coli and Salmonella typhimurium, and divided into a control group and two additive groups to determine the effect of an additive mixture on the changes in protein expression. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations of 0.5% or 1%. On the 1st day and after eight weeks of feeding, the body weight gain, food intake and serum GOT/GPT levels were examined. The GOT/GPT levels on the 1st day were similar in the three groups. However, after eight weeks of feeding, the GOT level was significantly lower in the additive treatment groups (0.5% and 1.0%). In addition, the changes in the spleen proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. A total of 31 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine spleens. Finally, 19 proteins were detected by MALDI-TOF/MS. Overall, the proteins detected are involved in a range of biological process, such as metabolic processes, biological processes, transport, carbohydrate metabolic processes, generation of precursors and energy. In conclusion, these results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the stress of microbial infections.
- Subjects
ESCHERICHIA coli disease prevention; ANALYSIS of variance; ANIMAL experimentation; BACTERIAL antigens; COMPUTER software; ELECTROPHORESIS; ESCHERICHIA coli diseases; FOOD additives; IMMUNOGLOBULINS; MASS spectrometry; ORAL drug administration; RESEARCH funding; SWINE; T-test (Statistics); PROTEOMICS; DATA analysis; SALMONELLA diseases; PREVENTION
- Publication
American Journal of Chinese Medicine, 2011, Vol 39, Issue 3, p477
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X1100897X