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- Title
The seed-borne Southern bean mosaic virus hinders the early events of nodulation and growth in Rhizobium-inoculated Phaseolus vulgaris L.
- Authors
López, Mariadaniela; Muñoz, Nacira; Lascano, Hernan Ramiro; Izaguirre-Mayoral, María Luisa
- Abstract
To simulate seed-borne virus transmission, a noninvasive protocol was designed to infect the radicle of germinating seeds, with 100% effectiveness. Preinfection of 24-h-old black bean (Phaseolus vulgaris L.) radicles by Southern bean mosaic virus (SBMV) followed by Rhizobium inoculation 48 h later caused a drastic reduction in root nodulation. Results were attributed to active virus replication within the elongating zone of the radicle at least 32 h before Rhizobium inoculation, which elicited severe anatomical malformations; an abnormal accumulation of apoplastic reactive oxygen species in the rhizodermis, cortex, inner cortical and endodermic root cells; the formation of atypical root hair tips and the collapse of 94% of the root hairs in the SBMV-preinfected radicles. Adult SBMV-preinfected plants showed exacerbated virus symptoms and 80% growth reduction ascribed to major virus-induced ultrastructural alterations in the nodules. The accumulation of ureides, αamino acids and total reducing sugars in the leaves and nodules of SBMVpreinfected plants are indicators of the hindering effects of SBMV infection on N2 fixation and ureide catabolism, causing N starvation. The exogenous addition of 1 or 4 μM naringenin, genistein or daidzein did not counteract the deleterious effects of SBMV preinfection on nodulation.
- Subjects
COMMON bean; SEED-borne phytopathogens; MOSAIC viruses; GERMINATION; RHIZOBIUM; VIRUS disease transmission
- Publication
Functional Plant Biology, 2017, Vol 44, Issue 2, p208
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP16180