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- Title
Development and validation of stability indicating RP-HPLC method for estimation of Garenoxacin Mesylate in tablet dosage form.
- Authors
Panchal, Ashwinkumar B.; Patel, Bhumi R.; Patel, Jaymin G.; Patel, Yogesh K.; Maurya, Udayshankar J.
- Abstract
A simple, rapid, economical, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) Stability indicating method for Estimation of GarenoxacinMesylate in tablet dosage form has been developed. RP-HPLC method was developed for estimation of Garenoxacinmesylate in tablet dosage form. In RP-HPLC method, Hypersil BDS C18 (250*4.6) mm & 5 μm particle size; thermo scientific column and 0.05 M Potassium dihydrogen phosphate pH-6.0 with 0.1 M sodium hydroxide: Methanol (30:70 v/v) was used as mobile phase. Retention time of Garenoxacinmesylate was found to be about 4.5 minute at 273 nm with a flow rate of 1.0 ml/min.Forced degradation study of Garenoxacinmesylate was performed by RP-HPLC method which includes Acid, Base, Oxidative, Photo and Thermal degradation. Results of degradation were found within limit. The method has been validated for linearity, accuracy and precision. Linearity was observed for range of 5-15 μg/mL. The percentage recovery obtained was found to be 100.29 ± 0.76. The proposed stability indicating RP-HPLC method is specific, linear, precise and accurate, so this method can be successfully applied for the estimation of Garenoxacin mesylate in the tablet dosage form.
- Subjects
HIGH performance liquid chromatography; DOSAGE forms of drugs; POTASSIUM dihydrogen phosphate; SODIUM hydroxide; DNA topoisomerase II; DNA topoisomerases
- Publication
International Journal of Pharmacy & Life Sciences, 2017, Vol 8, Issue 3, p5504
- ISSN
0976-7126
- Publication type
Article