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- Title
NANOSTRUCTURED BIOSENSORS BASED ON TYROSINASE-LANGMUIR-BLODGETT FILMS FOR THE DETECTION OF CATECHOL.
- Authors
Apetrei, Constantin; Dima, Ştefan
- Abstract
The control of molecular organization has been a crucial factor for the use of Langmuir-Blodgett films in biosensors, mainly because enzymes can be immobilized with preserved activity. In this work has been studied the inclusion of tyrosinase in hybrid Langmuir films of arachidic acid and gadolinium bisphthalocyanine. These organized mixed films of AA-GdPc2/Tyr could be transferred onto ITO and Pt electrodes as indicated by spectrometry and electrochemical measurements. Appreciably, the activity of the immobilized Tyr was significantly higher than in previous work in the literature, which allowed Tyr-containing LB films to be used as highly sensitive voltammetric sensors to detect catechol. Linear responses have been achieved up to 400 µM, with a detection limit of 2.1 µM (n=7) and sensitivity of 1.62 µA·µM-1·cm-2. Additionally, the Hill coefficient (h = 1.08) indicates cooperation of tyrosinase with GdPc2 that also acts as a catalyst. The exceptional functionality of the enzyme obtained using a biomimetic immobilization method, the electrochemical signal improvement caused by the gadolinium bisphthalocyanine mediator make this biosensor exceptionally appropriate for the detection of catechol.
- Subjects
PERFORMANCE of biosensors; PHENOL oxidase; NANOBIOTECHNOLOGY; CATECHOL; LANGMUIR isotherms
- Publication
Annals of the University Dunarea de Jos of Galati: Fascicle II, Mathematics, Physics, Theoretical Mechanics, 2011, Vol 34, p10
- ISSN
2067-2071
- Publication type
Article