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- Title
PD-1 up-regulation on CD4<sup>+</sup> T cells promotes pulmonary fibrosis through STAT3-mediated IL-17A and TGF-β1 production.
- Authors
Celada, Lindsay J.; Kropski, Jonathan A.; Herazo-Maya, Jose D.; Luo, Weifeng; Creecy, Amy; Abad, Andrew T.; Chioma, Ozioma S.; Lee, Grace; Hassell, Natalie E.; Shaginurova, Guzel I.; Wang, Yufen; Johnson, Joyce E.; Kerrigan, Amy; Mason, Wendi R.; Baughman, Robert P.; Ayers, Gregory D.; Bernard, Gordon R.; Culver, Daniel A.; Montgomery, Courtney G.; Maher, Toby M.
- Abstract
PD-1+TH17 cells enhance collagen-1 production from human lung fibroblasts. PD-1–expressing T cells prompt pulmonary fibrosis: Although T cells expressing programmed cell death-1 (PD-1) are sometimes described as exhausted, they are not too tuckered out to wreak havoc in a variety of settings. Celada et al. examined cells from patients with sarcoidosis or idiopathic pulmonary fibrosis and saw an increase in PD-1+CD4+ T cells relative to healthy controls. These cells were mostly TH17 cells and were able to induce fibroblasts to produce collagen in vitro. Blocking PD-1 in the coculture system prevented this induction and associated cytokine production from the T cells. The authors then demonstrated that blocking PD-1 in a mouse bleomycin model reduced fibrosis symptoms. Putting these cells to sleep may be a way to help patients with pulmonary fibrosis. Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic and immunologic investigations suggest common intersections between idiopathic pulmonary fibrosis (IPF), sarcoidosis, and murine models of pulmonary fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, and flow cytometric analysis of human and murine specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF lymphocytes. PD-1+CD4+ T cells with reduced proliferative capacity and increased transforming growth factor–β (TGF-β)/interleukin-17A (IL-17A) expression were detected in IPF, sarcoidosis, and bleomycin CD4+ T cells. PD-1+ T helper 17 cells are the predominant CD4+ T cell subset expressing TGF-β. Coculture of PD-1+CD4+ T cells with human lung fibroblasts induced collagen-1 production. Strikingly, ex vivo PD-1 pathway blockade resulted in reductions in TGF-β and IL-17A expression from CD4+ T cells, with concomitant declines in collagen-1 production from fibroblasts. Molecular analysis demonstrated PD-1 regulation of the transcription factor STAT3 (signal transducer and activator of transcription 3). Chemical blockade of STAT3, using the inhibitor STATTIC, inhibited collagen-1 production. Both bleomycin administration to PD-1 null mice or use of antibody against programmed cell death ligand 1 (PD-L1) demonstrated significantly reduced fibrosis compared to controls. This work identifies a critical, previously unrecognized role for PD-1+CD4+ T cells in pulmonary fibrosis, supporting the use of readily available therapeutics that directly address interstitial lung disease pathophysiology.
- Subjects
PULMONARY fibrosis; INTERLEUKINS; CELL membranes; T cells; FLOW cytometry; IMMUNOGLOBULINS; GENETICS
- Publication
Science Translational Medicine, 2018, Vol 10, Issue 460, p1
- ISSN
1946-6234
- Publication type
Article
- DOI
10.1126/scitranslmed.aar8356