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- Title
Enzymatic Resolution by a d-Lactate Oxidase Catalyzed Reaction for ( S)-2-Hydroxycarboxylic Acids.
- Authors
ShENg, Binbin; Xu, Jing; Ge, YongshENg; Zhang, Shuo; Wang, Danqi; Gao, Chao; Ma, Cuiqing; Xu, Ping
- Abstract
Oxidase-catalyzed kinetic resolution is important for the production of enantiopure 2-hydroxycarboxylic acids (2-HAs), which are versatile building blocks for the synthesis of many significant compounds. However, in contrast to that of ( R)-2-HAs, the production of ( S)-2-HA is challenging because of the lack of related oxidases. Herein, suitable enzymes were screened systematically through the analysis of numerous putative d-lactate oxidase sequences and identification of several required properties. Finally, a d-lactate oxidase from Gluconobacter oxydans 621H with advantageous characteristics, such as good solubility, broad substrate spectrum, and high stereoselectivity, was selected to resolve 2-HAs into ( S)-2-HAs. A variety of ( S)-2-HAs was produced successfully using this d-lactate oxidase with excellent enantiomeric excess values (>99 %). The presented screening criteria and approach for target biocatalysis suggested a guideline for the production of optically active chemicals such as ( S)-2-HAs.
- Subjects
ENZYMATIC analysis; LACTATES; HYDROXY carboxylic acids; CHEMICALS; CHEMICAL synthesis
- Publication
ChemCatChem, 2016, Vol 8, Issue 16, p2630
- ISSN
1867-3880
- Publication type
Article
- DOI
10.1002/cctc.201600536