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- Title
Efficacy of antioxidant and antimicrobial activity of whole porcine blood hydrolysates and its fractions under in-vitro conditions.
- Authors
Verma, Akhilesh K.; Chatli, Manish Kumar; Mehta, Nitin; Kumar, Pavan
- Abstract
Protein hydrolysates were recovered from porcine blood hydrolysate (PBH) using enzymatic hydrolysis viz. alcalase, trypsin and papain. The PBH were ultra-filtered by using molecular weight cut off, these PBH and different fractions were evaluated for antioxidant and antimicrobial activity. The PBH and fractions were assessed for antioxidant efficacy viz. 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2, 2-diphenyl-1-picrylhydrazyl and Ferric reducing antioxidant power assay and antimicrobial activity by zone of inhibition assay. All antioxidant parameters assessed for inhibition activity were observed significantly (P < 0.05) higher for whole PBH whereas among fractions (5–10 kDa), (1–5 kDa) for all hydrolysates had comparatively higher antioxidant efficacy. The results showed that PBH obtained from trypsin and its fractions have highest antioxidant activities. The antimicrobial efficacy was also higher for whole PBH than their respective fractions; however, the zone of inhibition varied significantly (P < 0.05) among different fractions. Results revealed that among enzymes trypsin and alcalase could produce peptides with comparatively higher antimicrobial activity for all tested microbes than papain. The results concluded that porcine blood hydrolysates can be fractioned to get lower molecular weight peptides of interest; however, for addition of whole PBH in meat products or other use could be more useful with regards to its antioxidant, antimicrobial activity and economic production. Natural food preservatives derived from blood can be an alternative for artificial additives widely used in food and the pharmaceutical industry. Hydrolysates obtained by enzymatic hydrolysis of porcine blood were assessed for their antioxidant and antimicrobial efficacy. The study revealed that porcine blood hydrolysate (whole and fractions) can be a potential source of natural preservatives for shelf-life extension in food.
- Subjects
PROTEIN hydrolysates; MEAT quality; ANTIOXIDANTS &; health
- Publication
Animal Production Science, 2018, Vol 58, Issue 11, p2084
- ISSN
1836-0939
- Publication type
Article
- DOI
10.1071/AN16804