We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Autophagy regulates the stemness of cervical cancer stem cells.
- Authors
Yi Yang; Li Yu; Jin li; Ya Hong Yuan; Xiao Li Wang; Shi Rong Yan; Dong Sheng Li; Yan Ding
- Abstract
Cancer stem cells (CSCs) are a rare population of multipotent cells with the capacity to self-renew. It has been reported that there are CSCs in cervical cancer cells. Pluripotency- associated (PA) transcription factors such as Oct4, Sox2, Nanog and CD44 have been used to isolate CSCs subpopulations. In this study, we showed that autophagy plays an important role in the biological behavior of cervical cancer cells. The expression of the autophagy protein Beclin 1 and LC3B was higher in tumorspheres established from human cervical cancers cell lines (and CaSki) than in the parental adherent cells. It was also observed that the basal and starvation-induced autophagy flux was higher in tumorspheres than in the bulk population. Autophagy could regulate the expression level of PA proteins in cervical CSCs. In addition, CRISPR/Cas 9-mediated Beclin 1 knockout enhanced the malignancy of HeLa cells, leading to accumulation of PA proteins and promoted tumorsphere formation. Our fndings suggest that autophagy modulates homeostasis of PA proteins, and Beclin 1 is critical for CSC maintenance and tumor development in nude mice. This demonstrates that a prosurvival autophagic pathway is critical for CSC maintenance.
- Subjects
CERVICAL cancer; STEM cells; AUTOPHAGY; EMBRYONIC stem cells; CANCER stem cells
- Publication
Biologics: Targets & Therapy, 2017, Vol 11, p71
- ISSN
1177-5475
- Publication type
Article
- DOI
10.2147/BTT.S134920