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- Title
2,4,3',4'-tetramethoxy-biphenyl induces apoptosis in MGC-803 cells through a mitochondrial/caspase pathway.
- Authors
Xin Sui; Zhizhong Sun; Weiwei An; Kai Cheng; Shougang Jiang; Xianfeng Gong
- Abstract
Anti-proliferative and apoptosis-inducing effects of 2,4,3',4'-tetramethoxybiphenyl (TMBP) on human gastric cancer MGC-803 cells were investigated. The molecular mechanisms of TMBP-mediated tumor cell death were detected by clonogenic assay, staining with Hoechst 33258, DNA fragmentation assay, Western blot analysis and flow cytometry assay. Studies on MGC-803 cells treated with TMBP showed that TMBP inhibited the proliferation of MGC-803 cells in a time- and dose-dependent manner. The induction of apoptosis by TMBP was accompanied by the loss of mitochondrial membrane potential (ΔΨm), cytochrome C release and activation of caspase cascade, resulting in the cleavage of some specific substrates for caspase-3 such as poly (ADPribose) polymerase (PARP). In conclusion, these findings showed that TMBP may induce the apoptosis of MGC-803 through a mitochondrial/caspase pathway, suggesting its possible use for treating human cancers.
- Subjects
BIPHENYL compound derivatives; ANTINEOPLASTIC agents; APOPTOSIS; MITOCHONDRIA; CASPASES; WESTERN immunoblotting; CELLULAR signal transduction
- Publication
Bangladesh Journal of Pharmacology, 2014, Vol 9, Issue 2, p235
- ISSN
1991-007X
- Publication type
Article
- DOI
10.3329/bjp.v9i2.18430