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- Title
Inner Anti-inflammatory Mechanisms of Petroleum Ether Extract from Melilotus suaveolens Ledeb.
- Authors
Lei Zhao; Jun-Yan Tao; Shu-Ling Zhang; Ran Pang; Feng Jin; Ji-Hua Dong; Yuan-Jin Guo
- Abstract
Abstract Summary  Melilotus suaveolens Ledeb is a species of traditional medical plant for treating inflammation-related disease. This study is to explore the inner anti-inflammatory mechanism on petroleum ether extract from Melilotus suaveolens Ledeb. Materials and Methods  Inflammatory cellular model was founded by intervention of lipopolysaccharide (LPS) on RAW264.7 cell line. Secretion of TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed. Results  The extract could not only reduce production of pro-inflammatory mediators by blocking NF-κB activation but promote release of anti-inflammatory mediator HO-1 significantly. The only active ingredient in the extract was coumarin and the concentration of coumarin in each 1 g extract was 0.27822 mg. Conclusion  Compared to Dexamethasone, the extract not only has similar effects on antagonizing pro-inflammatory mediators and cytokines but has effects on promoting production of anti-inflammatory mediators.
- Subjects
INFLAMMATORY mediators; ANTICOAGULANTS; CELL culture; MESSENGER RNA
- Publication
Inflammation, 2007, Vol 30, Issue 6, p213
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-007-9039-x