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- Title
Denitrification process of Casuarina root nodule endophyte Frankia.
- Authors
CHEN Hui; ZHU Cheng; LIN Hong-lian; MA Hongliang; YIN Yunieng; GAO Ren
- Abstract
To examine the characteristic of denitrification in Frankia, a symbiotic nitrogen-fixing microbe associated with non-leguminous plants, and its role as a N2 O source or sink, Casuarina root nodule endophyte Frankia was isolated using sectioning method, which was then purely cultured to investigate the denitrification process under NO3-addition. The results showed that after addition of NO3-to the medium under anaerobic condition, the concentration of NO3-decreased with time, while the concentrations of NO2-and N2 O initially increased and then decreased over time. Key denitrification genes and nitrogenase gene were detected at 26 h, 54 h and 98 h during incubation. Abundances of these genes significantly delivered among each other, and their dynamics were asynchronous. Redundancy analysis of the effect of NO3-, NO2-, N2O concentrations on abundances of denitrification genes and nitrogenase gene indicated that 81.9% of' the total variation in gene abundances could be explained by the first two axes. Frankia had a denitrifying activity under anaerobic condition, with denitrification genes, including nitrous oxide reductase gene (nosZ), being identified. Our results suggested that Frankia possessed a complete denitrification pathway and the ability of N2O reduction under anaerobic condition.
- Subjects
ROOT-tubercles; DENITRIFICATION; CASUARINA; DENITRIFYING bacteria; NITROUS oxide; NITROGENASES
- Publication
Yingyong Shengtai Xuebao, 2023, Vol 34, Issue 4, p1109
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202304.005