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- Title
Engineering nitrogen use efficiency with alanine aminotransferase.
- Authors
Good, Allen G.; Johnson, Susan J.; De Pauw, Mary; Carroll, Rebecka T.; Savidov, Nic; Vidmar, John; Lu, Zhongjin; Taylor, Gregory; Stroeher, Virginia
- Abstract
Nitrogen (N) is the most important factor limiting crop productivity worldwide. The ability of plants to acquire N from applied fertilizers is one of the critical steps limiting the efficient use of nitrogen. To improve N use efficiency, genetically modified plants that overexpress alanine aminotransferase (AlaAT) were engineered by introducing a barley AlaAT cDNA driven by a canola root specific promoter (btg26). Compared with wild-type canola, transgenic plants had increased biomass and seed yield both in the laboratory and field under low N conditions, whereas no differences were observed under high N. The transgenics also had increased nitrate influx. These changes resulted in a 40% decrease in the amount of applied nitrogen fertilizer required under field conditions to achieve yields equivalent to wild-type plants.
- Subjects
AGRICULTURAL productivity; NITROGEN; ALANINE aminotransferase; CROPS; FERTILIZERS; BARLEY; TRANSGENIC plants; CROP yields; PLANT genetic engineering
- Publication
Canadian Journal of Botany, 2007, Vol 85, Issue 3, p252
- ISSN
0008-4026
- Publication type
Article
- DOI
10.1139/B07-019