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- Title
A molecular mechanism for the low-pH stability of sialidase activity of influenza A virus N2 neuraminidases<sup>1</sup><FN ID="FN1"><NO>1</NO>Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB101671, AB101672, AB101673, AB101674 and AB101675.</FN>
- Authors
Takahashi, Tadanobu; Suzuki, Takashi; Hidari, Kazuya I.-P. Jwa; Miyamoto, Daisei; Suzuki, Yasuo
- Abstract
Four human pandemic influenza A virus strains isolated in 1957 and 1968, but not most of the epidemic strains isolated after 1968, possess sialidase activity under low-pH conditions. Here, we used cell-expressed neuraminidases (NAs) to determine the region of the N2 NA that is associated with low-pH stability of sialidase activity. We found that consensus amino acid regions responsible for low-pH stability did not exist in pandemic NAs but that two amino acid substitutions in the low-pH-stable A/Hong Kong/1/68 (H3N2) NA and a single substitution in the low-pH-unstable A/Texas/68 (H2N2) NA resulted in significant change in low-pH stability.
- Subjects
INFLUENZA; MOSAICISM
- Publication
FEBS Letters, 2003, Vol 543, Issue 1-3, p71
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00403-4