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- Title
Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence
- Authors
Sano, Ken-Ichi; Maeda, Kayo; Oki, Manami; Maéda, Yuichiro
- Abstract
We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus-infected insect cells. This 21 bp sequence element is derived from a 5′ untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20- and seven-fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A-rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus-based expression system.
- Subjects
BACULOVIRUSES; INSECT cell biotechnology
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p143
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03659-1