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- Title
A cytochrome c encoded by the nar operon is required for the synthesis of active respiratory nitrate reductase in Thermus thermophilus
- Authors
Zafra, Olga; Ramírez, Sandra; Castán, Pablo; Moreno, Renata; Cava, Felipe; Vallés, Cristina; Caro, Eddy; Berenguer, José
- Abstract
A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus. NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The α and δ subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus.
- Subjects
CYTOCHROME c; OPERONS; ENZYMES; NITRATES
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p99
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02953-8