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- Title
The Detection of Osthole and Application of Cell Imaging Based on Nitrogen-Doped Carbon Dots.
- Authors
Wang, Ning; Yang, Xuefang; Wang, Haojiang; Xie, Liping; Jia, Weihua; Bian, Wei; Choi, Martin M. F.
- Abstract
A fluorescence probe has been synthesized for the detection of osthole using the nitrogen-doped carbon dots (NCDs) as shown in Fig. 1. The NCDs were fully characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, the NCDs fluorescence probe was highly selective and sensitive to osthole. The linear response range for osthole was 5.0–75 μ M with a detection limit of 38 nM. The mechanism of the interaction of osthole and NCDs was discussed. The fluorescence probe has been applied to the analysis of biological samples. The as-synthesized NCDs with high fluorescence intensity, low toxicity and good biocompatibility were applied to cell imaging. NCDs were successfully synthesized by a microwave-heating method using citric acid and urea as the raw materials. The as-synthesized NCDs were spherical in shape and distributed uniformly. Based on the fluorescence quenching of NCDs by osthole under the optimal experimental conditions, a fluorescence probe was constructed for detecting osthole. The fluorescence probe with high fluorescence intensity, low toxicity and good biocompatibility has been applied to the detection of osthole of biological samples.
- Subjects
CELL imaging; FOURIER transform infrared spectroscopy; X-ray photoelectron spectroscopy; FLUORESCENCE quenching; TRANSMISSION electron microscopy
- Publication
NANO, 2020, Vol 15, Issue 2, pN.PAG
- ISSN
1793-2920
- Publication type
Article
- DOI
10.1142/S1793292020500253