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- Title
A Simple, Low Cost, Sensitive, and Portable Electrochemical Immunochromatography Sensing Device to Measure Estrone-3-Sulfate.
- Authors
Iwasaki, Wataru; Kataoka, Chiwa; Sawadaishi, Kazuyuki; Suyama, Keitaro; Morita, Nobutomo; Miyazaki, Masaya
- Abstract
In livestock production, point-of-care testing (POCT) technology that enables easy on-site analysis of sex hormones is desired to improve reproductive efficiency. In this context, low-molecular-weight endogenous steroids are particularly important for perinatal management. Therefore, we attempted to use a simple method that combines electrochemical techniques with immunochromatography to measure estrone-3-sulfate (E1S), one of the low-molecular-weight endogenous steroids that is an estrogen ester. The limit of detection (LOD) for E1S achieved by electrochemical immunochromatography was 570.5 ng/mL, which was one to two orders of magnitude lower than that of small molecule compounds analyzed by other POCT techniques (Primpray et al., Anal. Chim. Acta, 2019). In addition, it was indicated by a colorimetric analysis that the sensitivity of the electrochemical immunochromatographic technique could be enhanced by improving the method of application of the antibodies on the nitrocellulose membrane and the contact between the electrochemical detector and the nitrocellulose membrane.
- Subjects
ELECTROCHEMICAL sensors; SMALL molecules; COLORIMETRIC analysis; POINT-of-care testing; DETECTION limit; ESTROGEN
- Publication
Sensors (14248220), 2020, Vol 20, Issue 17, p4781
- ISSN
1424-8220
- Publication type
Letter
- DOI
10.3390/s20174781