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- Title
A Comparative and Critical Analysis for In Vitro Cytotoxic Evaluation of Magneto-Crystalline Zinc Ferrite Nanoparticles Using MTT, Crystal Violet, LDH, and Apoptosis Assay.
- Authors
de la Fuente-Jiménez, Juan Luis; Rodríguez-Rivas, César Iván; Mitre-Aguilar, Irma Beatriz; Torres-Copado, Andrea; García-López, Eric Alejandro; Herrera-Celis, José; Arvizu-Espinosa, María Goretti; Garza-Navarro, Marco Antonio; Arriaga, Luis Gerardo; García, Janet Ledesma; García-Gutiérrez, Domingo Ixcóatl; Dehesa, Alejandro Zentella; Sharma, Ashutosh; Oza, Goldie
- Abstract
Zinc ferrite nanoparticles (ZFO NPs) are a promising magneto-crystalline platform for nanomedicine-based cancer theranostics. ZFO NPs synthesized using co-precipitation method are characterized using different techniques. UV-visible spectroscopy exhibits absorption peaks specific for ZFO. Raman spectroscopy identifies Raman active, infrared active, and silent vibrational modes while Fourier transforms infrared spectroscopic (FTIR) spectra display IR active modes that confirm the presence of ZFO. X-ray diffraction pattern (XRD) exhibits the crystalline planes of single-phase ZFO with a face-centered cubic structure that coincides with the selected area electron diffraction pattern (SAED). The average particle size according to high-resolution transmission electron microscopy (HR-TEM) is 5.6 nm. X-ray photoelectron spectroscopy (XPS) signals confirm the chemical states of Fe, Zn, and O. A superconducting quantum interference device (SQUID) displays the magnetic response of ZFO NPs, showing a magnetic moment of 45.5 emu/gm at 70 kOe. These ZFO NPs were then employed for comparative cytotoxicity evaluation using MTT, crystal violet, and LDH assays on breast adenocarcinoma epithelial cell (MCF-7), triple-negative breast cancer lines (MDA-MB 231), and human embryonic kidney cell lines (HEK-293). Flow cytometric analysis of all the three cell lines were performed in various concentrations of ZFO NPs for automated cell counting and sorting based on live cells, cells entering in early or late apoptotic phase, as well as in the necrotic phase. This analysis confirmed that ZFO NPs are more cytotoxic towards triple-negative breast cancer cells (MDA-MB-231) as compared to breast adenocarcinoma cells (MCF-7) and normal cell lines (HEK-293), thus corroborating that ZFO can be exploited for cancer therapeutics.
- Subjects
ZINC ferrites; NANOMEDICINE; GENTIAN violet; FACE centered cubic structure; TRIPLE-negative breast cancer; X-ray photoelectron spectroscopy; SUPERCONDUCTING quantum interference devices
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 16, p12860
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms241612860