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- Title
Efficient co‐cultivation of human fibroblast cells (HFCs) and adipose‐derived stem cells (ADSs) on gelatin/PLCL nanofiber.
- Authors
Ranjbar‐Mohammadi, Marziyeh; Mousavi, Elham; Mostakhdem Hashemi, Mohammad; Abbasian, Mahdi; Asadi, Jahanbakhsh; Esmaili, Ehsan; Fesharaki, Mehrafarin; Asadi, Pouyan; Arab‐Bafrani, Zahra
- Abstract
In this study, we investigated whether the nanofibers produced by natural‐synthetic polymers can probably promote the proliferation of co‐cultured adipose‐derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L‐lactide co‐ɛ‐caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real‐time polymerase chain reaction were applied to assess the proliferation of co‐cultured ADSs/HFCs and the collagen type I and III synthesis, respectively. The concentrations of two cytokines including fibroblast growth factor‐basic and transforming growth factor‐β1 were also measured in culture medium of co‐cultured ADSs/HDCs using enzyme‐linked immunosorbent assay assay. Actually, nanofibers exhibited proper structural properties in terms of stability in cell proliferation and toxicity analysis processes. Gel/PLCL nanofiber promoted the growth and the adhesion of HFCs. Our results showed in contact co‐culture of ADSs/HFCs on the Gel/PLCL nanofiber increased cellular adhesion and proliferation synergistically compared to non‐coated plate. Also, synthesis of collagen and cytokines secretion of co‐cultured ADSs/HFCs on Gel/PLCL scaffolds is significantly higher than non‐coated plates. To conclude, the results suggest that Gel/PLCL nanofiber can imitate physiological characteristics in vivo and enhance the efficacy of co‐cultured ADSs/HFCs in wound healing process.
- Publication
IET Nanobiotechnology (Wiley-Blackwell), 2020, Vol 14, Issue 1, p73
- ISSN
1751-8741
- Publication type
Article
- DOI
10.1049/iet-nbt.2019.0278