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- Title
Structural Insights into Iron Ions Accumulation in Dps Nanocage.
- Authors
Chesnokov, Yury; Mozhaev, Andrey; Kamyshinsky, Roman; Gordienko, Alexander; Dadinova, Liubov
- Abstract
Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate clusters of ferric ions in its nanocages, thus providing the bacterium with physical and chemical protection. Here, cryo-electron microscopy was used to study the accumulation of iron ions in the nanocage of a Dps protein from Escherichia coli. We demonstrate that Fe2+ concentration in the solution and incubation time have an insignificant effect on the volume and the morphology of iron minerals formed in Dps nanocages. However, an increase in the Fe2+ level leads to an increase in the proportion of larger clusters and the clusters themselves are composed of discrete ~1–1.5 nm subunits.
- Subjects
IRON ions; DNA-binding proteins; COMPLEX ions; BACTERIAL DNA; IRON; FERRITIN
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 10, p5313
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23105313