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- Title
亚甲蓝与β-胡萝卜素双褪色法在大豆种子脂氧酶缺失 育种上的应用研究.
- Authors
王先虚; 范慧明; 藕冉; 王磊; 王遂; 姜妍; 王绍东
- Abstract
The development of screening and identification methods for three isozyme lacking characteristics of lipoxygenase(lox-1,2,3)is significance to the breeding and phenotypic identification of odorless soybean with lipoxygenases lacking in F2 soybean seeds. In this study, methylene blue and β-carotene fading reactions at different pH values of three isoenzymes Lox-1,2,3 were used to accurately identify four phenotypes of Lox-1, 2, 3 wild type, Lox-1, 2, 3 free, Lox-1, 2 free, and Lox-3 free in F2 generation by trace sampling. The identification had no impacts on breeding of the selected individuals with target traits and could provide technical support for breeding and phenotypic identification of new soybean varieties with null lipoxygenases deficiency.
- Subjects
LIPOXYGENASES; ISOENZYMES; SEEDS; PHENOTYPES
- Publication
Chinese Journal of Oil Crop Sciences, 2022, Vol 44, Issue 5, p1123
- ISSN
1007-9084
- Publication type
Article
- DOI
10.19802/j.issn.1007-9084.2021235