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- Title
Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents.
- Authors
Goh, Myeong-Jin; Lee, Hae-Kwang; Liang Cheng; Kong, De-Yun; Yeon, Jae-Ho; He, Quan-Quan; Cho, Jun-Cheol; Na, Yong Joo
- Abstract
The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment.
- Subjects
HYPERPIGMENTATION; MELANOCYTES; PHENOL oxidase; MELANOGENESIS; SKIN physiology; ENZYME inhibitors; LABORATORY mice; GENE expression; PREVENTION
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 1, p1655
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms14011655