We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transposon-mediated random gene disruption with moderate halophilic bacteria and its application for halophilic bacterial siderophore analysis.
- Authors
Matsui, Toru; Nishino, Tomohiko
- Abstract
Analytical conditions using chromo azurol S was validated for quantification of siderophore in aqueous samples, followed by the characterization of siderophore derived from newly isolated moderately halophilic bacteria. Conditions with good linearity between the absorbance and the siderophore concentration were obtained at a siderophore concentration less than 20 µM, in the wavelength range between 630 and 660 nm with developing time for at least 2 h. Of the halophilic bacteria isolated from Tunisian soil, Halomonas sp., namely strain 21a was selected as siderophore producing halophiles. The strain produced siderophore significantly in the absence of iron in minimal medium. Siderophore-deficient mutant, namely IIa10, of the strain 21a was obtained from gene disruptant library constructed using transposon complex by electroporation. Genomic sequence analysis of the mutant IIa10 revealed that the transposon-inserted gene was TonB-dependent receptor.
- Subjects
FUNGUS-bacterium relationships; HALOBACTERIUM; PROKARYOTES; HALOPHILIC organisms; HALOMONAS (Bacteria); SIDEROPHORES; TRANSPOSONS; BACTERIA
- Publication
Journal of Basic Microbiology, 2016, Vol 56, Issue 12, p1354
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201600121